ASTM D – 15 Standard Test Method for Analysis of Aqueous Extractable Protein in Latex, Natural Rubber, and Elastomeric Products Using the Modified. RRIM (MS P) and the ASTM (D ) modified Lowry tests, were examined and their relationship studied. Total extractable proteins, EPRRiMt. ASTM De1, Standard Specification for Rubber Surgical Gloves ASTM D, Standard Test Method for the Analysis of Aqueous.

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A previous paper on cleaning latex films with fumed silica5 presented protein results derived by ASTM D Some of the proteins can be more harmful than others.

Many efforts have been made to remove the proteins. Enter your account email address to request a password reset: Natural Rubber Science and Technology, A.

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Powder-free latex gloves and other methods of reducing protein content Latex gloves are among the most commonly used critical latex articles. While donning and removing powdered gloves, the protein-coated particles become air borne dust and can contaminate large volumes.

Link to Active This link will always route to the current Active version awtm the standard. It is suggested by R. An additional advantage is enhancing latex viscosity resulted only with fumed silica B. Thompson et al9 that there is a relationship between hydration and compromised barriers in some surgical gloves.


A precision and bias statement shall be reported at the end of a test method. Historically latex gloves are powdered for two reasons. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard. Adkinson, Journal of Allergy and Clinical Immunology, 98, About 10 percent of medical field employees dealing with dipped rubber products suffer from this allergy.

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Hamilton, Journal of Allergy and Clinical Immunology, 96, However, traces still may remain and cause skin reactions. Historical Version s – view previous versions of standard. Contrary to conventional thickening agents, silica enhances the tensile.


After displacement, the protein become mobile and easier to dispose off. There were no books found for the applied search filters. This problem has become more apparent in the last several years due to the increased use of rubber gloves for infectious diseases protection.

Latex Protein Analysis | Akron Rubber Development Laboratory

The proteins probably would stay adsorbed on the latex particles throughout the compounding process. However, the low silica concentrations necessary make this a cost-effective process for addressing protein allergy concerns. This problem has been publicized in the media and described in the literature.

A large number estimated in the order of magnitude of 10 percent in the U. Print 1 2 3 4 5 page sstarting from page current page. Summary and conclusions Cost and process advantage of latex dipped with fumed silica Presently, latex goods with relatively low protein content are being manufactured by various complicated methods.

It is reported that surgical gloves, long assumed effective barriers against blood-born pathogens, may not be effective as originally thought and, further, improperly manufactured gloves may cause a serious threat to healthcare workers and patients.

Other advantages are an increase in strength, stability and transport properties. Mechanisms of protein cleaning by fumed silica—displacement or attachment The mechanism by which dispersed fumed silica reduces protein content in the latex has not been scientifically established. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

It is thought that proteins in the glove are being washed out and replaced by water molecules, creating diffusible channels through which small diameter species, like the HIV virus, could pass. D Specification for Rubber Examination Gloves. Proteins in the ast, migrate to the surface and penetrate through the skin. Washing is minimal and since the latex is clean, there is no need for costly off d512 post treatments. It is suggested that the silica attaches itself to the surface of the latex particle substituting the protein molecules and displacing them, see Fig.


This paper suggests a way to eliminate the proteins with a simple, elegant and inexpensive method c5712 fumed silica additives.

Other methods for protein removal treatment include passing the latex through special ion exchange resins6 or altering the protein structure with protease enzymes. Today, some 12, ASTM standards are used around d57112 world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence.

ASTM International – Standard References for ASTM D – 15

It is well known that proteins can transfer from the latex to the powder. Akron Rubber Development Laboratory, Inc. November 20, Content source: The following editions for this book are also available However, the 20 ppm proteins left, may happen to be harmful and the latex status as far as allergen content did not change at all.

D571 is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

Examples of test methods include, asstm are not limited to: For example, someone can develop a process for latex protein cleaning, submit the clean latex to ASTM D test and find that the protein x5712 was reduced from ppm to 20 ppm.

Latex Protein Analysis

FORMAT Single User Subscription Site License Subscription BOOKS All Books PACKAGES All Packages EDITION to PUBLISHER. Extensive washing and film integrity As stated previously, powder-free latex gloves are produced by washing the powder away with water. Please login to your authorized staff account to use this feature.

Water soluble proteins are extracted in a buffer solution and then precipitated to concentrate them and also to separate them from water soluble substances that may interfere with the determination. It was found that the addition of fumed silica to latex compounds helps in manufacturing of cleaner latex goods with lower protein d572.